目的 了解厦门地区学龄前儿童细胞色素P450 3A酶(CYP3A)及分布活性特征。方法 用氢化可的松(HC)非侵入性探针法, 利用高效液相色谱(HPLC)测定尿液中6β-羟基氢化可的松(6β-OHC)、氢化可的松(HC)的浓度, 以两者的浓度比定量表示CYP3A酶活性。结果 287名儿童酶活性呈左偏态分布, 中位数为2.61, 四分位数间距为3.97。结论 不同性别间酶活性水平无显著性差别;随着年龄的升高, 酶活性水平呈现降低的趋势(P<0.05), 与年龄具有相关性;3岁组明显高于4岁组(P<0.05)。
Abstract
OBJECTIVE To investigate the CYP3A activity and its characteristics of distribution in the pre-school children in Xiamen. METHODS Using hydrocortisone as a noninvasive probe, urine concentrations of hydrocoritisone ydrocortisone and 6β-hydroxycortisol were determined by HPLC method.The urinary ratio of 6β-hydroxycortisol/ hydrocortisone was used to quantify CYP3A activity. RESULTS The CYP3A activity in the 287 healty children showed a skewed to the left distribution.The median value was 2.61 and the value of quartile range was 3.97. CONCLUSION There is no significant difference in CYP3A activity in gender. With increasing of ages, the CYP3A activity shows the decreasing tendency.The results show a statistic correlation between the ages and CYP3A activity.The value of CYP3A activity in age 3 group is higher than age 4 group.
关键词
CYP3A /
氢化可的松 /
6β-羟基氢化可的松 /
高效液相色谱法 /
学龄前儿童
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Key words
CYP3A /
hydrocortisone /
6β-hydroxycortisol /
HPLC /
children at pre-school age
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中图分类号：
R969.1
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参考文献
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脚注
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基金
厦门市科学技术局(3502Z20094018)资助
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